How To Tissue Propagate Plants Easily

In general, one can understand how to tissue propagate plants in four stages. This is basing on the micropropagation guide by the University of Florida, University of Kentucky, and Texas A&M University. It can be overwhelming for any gardener to learn this asexual propagation method because of its requirements and detailed steps. 

Still, tissue propagation is a useful solution if you have limitations with other techniques, and it also opens so many potentials for gardeners. There’s even a growing interest nowadays in banana propagation using this method because of its productivity. In a sense, being that these plants started in the laboratory, it’s also a viable consideration to grow them in a greenhouse until they are ready for outdoors. 


How To Tissue Propagate Plants Easily

How To Tissue Propagate Plants Successfully

Do note that there are many methods on how to tissue propagate plants. You can check out the more detailed and technical guide of the American Phytopathological Society for more information. Nonetheless, the four stages below are the basic concept of tissue propagation, and understanding it should help you learn more technical procedures quickly.


Stage #1

The first stage of micropropagation is the establishment, where you will sterilize the explant. Remember that what makes tissue propagation trickier than other methods is that everything should be clean and sterilized. To begin shoot multiplication, the explant should undergo sterilization with detergent and bleach or alcohol, and fungicide. 


Stage #2

After the establishment comes, the second stage is called shoot multiplication. From the name itself, you’re aiming to create many shoots from the explant. This is where you will notice how high the number of shoots produced in tissue propagation is compared to other methods. 

To do so, take a portion of the culture and place it on a sterile paper towel. This will serve as your subculture, where you can cut smaller pieces with a scalpel and forceps. Transfer them to another jar and use cytokinins to induce shoot formation. 


Stage #3 

The third stage of tissue propagation is when the shoots you’ve produced earlier will root to create a new plantlet. It’s typical to apply auxin onto the micro-cuttings to encourage root formation, either in vitro or ex vitro. The former is perhaps the most preferred method to create plants that will adapt easily to a normal environment. 

The technique itself is simple, where you handle the cuttings with forceps and then insert them in the rooting substrate. And besides, periodic spraying is vital to keep the cuttings from drying. 


Stage #4

The final method of tissue propagation is where you’ll acclimate the rooted plantlets. As briefly mentioned in the introduction, a common issue with plants from this method is they’ll need acclimation in a controlled environment before planting. You can use a greenhouse or a closed polyethylene tent for the plantlets and have them intermittently misted and gradually moved somewhere open-air with reduced humidity and light.


What Do You Need For Tissue Propagation?

Now that you learn the concept of this propagation method, the next step is to know the requirements and equipment types you must have to do the process successfully. First, you need an area that you can easily control and keep sterilized. This is why you might need an autoclave for disinfection, but a microwave or pressure cooker should also work well for smaller gardens. 

Next, the items you’ll need in addition to a disinfecting material are cleaners like bleach and hydrogen peroxide. And for the containers themselves, some people use recycled glass bottles, but you can always use other containers such as test tubes and pots depending on the step.


What Are The Requirements For Tissue Propagation?

Tissue propagation is somewhat meticulous. As discussed earlier, sanitation is vital from the plants, materials, and location. You should also check yourself to avoid drawbacks from contamination when culturing, and much like other propagation methods, it’s crucial to use a healthy plant. 

You will need a box or an old aquarium to create an environment with clean air for the environment and medium. You will also need to prepare and sterilize the growing medium and ensure that it will be stable and provide the plants’ nutrients. There are different mediums common for tissue propagation, such as agar. 



There will be instances where, as a gardener, you need to go out of the norm and expand your knowledge with other propagation methods. One of them is learning how to tissue propagate plants, which can be useful and beneficial in the ever-changing gardening scene. The process itself can be meticulous and have requirements you need to follow, but you can understand it better in four stages. 

In general, tissue propagation is composed of establishment, multiplication, rooting, and acclimatization. Throughout this propagation method, everything needs to be sterile and stable to guarantee success. And as you have read, the final stage, which is acclimatization, is necessary to prepare the “lab-grown” plants for a typical growing environment. 

By reading this guide and other detailed information from reliable sources, tissue propagation can be a DIY procedure. You can always get creative with the materials, and the benefits you’ll get include a high yield of quality plants. The main takeaway with tissue propagation or micropropagation is to practice sanitation and diligence throughout. 


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How To Prevent Root Rot In Hydroponics: 3 Useful Tips

If you’re a newbie gardener who’s looking to find ways to hone your skills, you’d want to learn how to prevent root rot in hydroponics even before this problem affects your plants.

Hydroponics can be advantageous to crops in more ways than one. However, it also comes with risks of diseases, such as root rot, which can be destructive or even lethal to your plants.

Unfortunately, there are no effective methods to recover the wilted parts that were affected by the root rot once it hits your plants. The only thing you can do if you do not want this catastrophe to befall your crops is to prevent it before it happens. Read on to learn more about this subject.


What is Root Rot?

Root rot is a disease that attacks the plant roots and causes them to suffer decay. This usually happens when a lack of oxygen supply occurs in the substrate.

To give you an idea, think about plant roots that are submerged in water that only has a little oxygen in it. Over time, the plant suffocates and dies.

Aside from rot and decay, this disease also leads to the proliferation of fungi that are naturally present in the soil. These include Rhizoctonia, Alternaria, Pythium, Botrytis, Fusarium, or Phytophthora. As soon as fungi colonies start to grow, they tend to target the weakened roots and infect your precious plant babies.

Once the plant becomes infected, they won’t be able to take in what they need to grow – water, oxygen, and other nutrients. When this happens, it won’t be long before the plant dies.


What is Hydroponics?

In case you’re not aware, the term hydroponic is derived from a Latin word that means “working water”. To put it simply, hydroponics is an art that involves growing various types of plants without soil. If you’re like most people, the first thing that comes to mind when somebody talks about hydroponics would be a picture of plants with roots suspended into the water without using any type of growing medium.


Avoiding Root Rot in Hydroponic Systems

Detecting and identifying root rot can be tricky. When your plants get infected, their leaves and roots gradually wither until the whole crop itself dies from the lack of nutrients, which is a common symptom of many diseases.


What causes root rot in hydroponics?

One of the requirements in hydroponics systems is oxygen. Without it, your plants are basically on the road to death. On the other hand, lack of such is one of the major triggers for root rot, and it must be avoided at all costs.

Just like when planting in soil, you loosen up the ground so that your plants’ roots can have their required intake of oxygen. That is the case for crops grown in aqueous solutions as well. If they cannot breathe, they would not be able to grow.

Another agent for root rot is the temperature. The last thing you would want in your system are parasites that leech nutrients intended for your plants and infect the water during the process. In common terms, these fungi are called molds.

One of the best breeding grounds for these is warm and moist areas. For this reason, if the water temperature inside your reservoir is high, then you are susceptible to it. Something as minor as letting the solutions exposed to sunlight can already be a risk factor.


3 Useful Tips on How to prevent root rot in hydroponics

There is good news! Root rot in hydroponics can be prevented! Just follow these tips:

Tip#1: Use the right air pump

If you do not want root rot to affect your plants, you merely have to avoid its causes. If you need oxygen, keep the water bubbling by providing an air pump of appropriate size, and also give importance to proper ventilation in the room.


Tip #2: Maintain the temperature

The temperature should be maintained within the 70 to 80 degrees F range. Get rid of any materials that can make your system vulnerable to infections, and make sure not to disturb your crops while they are trying to grow.


Tip #3: Get rid of the rotten parts

However, if you failed in preventing the disease, then the rotten parts should be removed immediately. Cut them off as there is no chance of reviving them, and focus on the potential new growth instead. Fix your hydroponics system and eliminate the risks.


Why Give Greenhouse Gardening a Try?

Greenhouse gardening offers numerous benefits to greens aficionados who dare to take their gardening experience to the next level. Aside from acting as a shield against the effects of inclement weather, a mini, hobby, or semi-pro greenhouse can also serve as a protective layer that keeps harmful bugs and critters at bay.

What’s more, its enclosed structure allows you to control your plants’ growing conditions including the temperature, light, moisture, and ventilation of the greenhouse’s internal environment. With a controlled environment, you’ll be able to extend growing seasons and grow plants that aren’t native to your area.



No matter how well-informed you are about how to prevent root rot in hydroponics, you cannot completely eradicate the risks. Therefore, to avoid the worst-case scenario, you should be prepared to sacrifice the infected for the sake of others. While you’re at it, consider trying your hand at greenhouse gardening as well.


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